Table of content
Evolution over the weeks
- Radical Mycology A to Z, Peter McCoy and Natassja Noell
- Mycelium running - how mushrooms can help save the world, Paul Stamets
- Growing Gourmet and medicinal mushrooms, Paul Stamets
- Fungi in bioremediation, G.-M. Gadd
- Psilocybin - Magic mushroom grower's guide, O.T. OSS and O.N. OERIC
- The mushroom cultivator - a practical guide to growing mushroom at home, Paul Stamets and J.S. Chilton
- Mycoremediation - fungal bioremediation, Harbhajan Singh
- Mycorrhizal biotechnology, D. Thangadurai, C.A. Busso, M. Hijri
- Cordyceps Cultivation handbook, William Padilla-Brown
- Fungi Materials Manual by Elise Elsacker (researcher), Kristel Peters (shoe designer), Winnie Poncelet (bio-engineer) from BioFab Forum
- Mushroom cultivation manual for the small mushroom entrepreneur
- Mycelium Material Study, Studio MUR MUR Chicago
- Nature: Collaborations in Design, Cooper Hewitt Design Triennial co-organized with Cube design museum
- The Mushroom at the End of the World, Anna Lowenhaupt Tsing
- Schimmel het plastic van de toekomst
- Paul Stamets: Mushroom Magic
- Paul Stamets: 6 ways mushrooms can save the world
- Fantastic Fungi
- Atelier Luma FR, Arles
- Eric Klarenbeek NL
- Maartje Dros NL
- Krown NL, Hedel
- Julian Hespenheide GE (Biotic Explorers Research Group)
- Maurizio Montalti NL, Amsterdam
- Ecovative Design Green Island, NY
- The Growing Pavilion NL
- The Growing Pavilion Atlas
- Huis Veendam Bio-Laminates NL, Groningen
- Local Forms A material research and adventure into mycelium
- Mykor Design UK
- Tree-shaped structure shows how mushroom roots could be used to create buildings
- the potential of mushrooms
- Blast studio 3D printed mycelium in UK, London
- Natura UK, "work with the ecosystem by redesigning current biosystems"
- BioHm UK "multi-award-winning research and development led, bio-manufacturing company. We allow nature to lead innovation, to revolutionise construction and create a healthier, more sustainable, built-environment"
Suppliers and recipes
- BioFab Forum
- BioFabForum: Mycelium Manual
- Ann Miller's Speciality Mushrooms Ltd (UK)
- krown e-shop (NL)
- Mycophilia For the love of fungi (NL)
- Mycelia Mycelium for professional (BE)
- CNC Exotic Mushrooms (NL)
- Cultivar Seitas (ES)
- Low-Tech Lab: mycelium's objects
- Low-Tech Lab: Pleurotes culture
- Conor Croasdell (former intern at Waag) → see his project a body heat incubator
- Candyce Dryburgh (former intern at Waag and founder of local forms)
- Inga Tilda (former intern at mediamatic)
- Yaël (researcher at TU Delft)
- Wasabii (researcher at TU Delft)
Tempeh bowl ●
→What is tempeh and how to make some
→Why create a composite material with Mycelium
First shape try
Before the fermentation process, I put the preparation in a bowl against the edges and I put a smaller bowl above. I wrapped it up with plastic film and made holes with a knife.
As you can see on the picture, the mycelium didn't grow in the bottom of the bowl because because it didn't have enough air.
I wanted to do some growth test with mycelium so I inoculate 3 types of mycelium in 4 types of nutrient medium.
|.||Schyzophilum||Grey Oyster||Tempeh starter|
Make and sterilise the medium
Coffee grounds are sterilised by the process of making coffee (when the boiled water pass into the coffee) so I directly took some coffee ground after making me a coffee and I spread it in 3 sterile petri dishes. Then I let them cool down to room temperature.
I crushed dried dog kibble and I spread them in 3 glass petri dishes that I reserved.
I prepared a pot with:
- 250ml distilled water
- 0,75g of Malt extract
- 0,75g of Yeast extract
- 1,25g of Peptone
- 2,5g of Glucose
I mixed it and I reserved.
(The recipe come from the biofabforum )
I mixed 7g of agar powder with 250ml of distilled water in a pot and I reserved.
(The recipe was on the agar powder box)
Autoclaving with pressure cooker
- I first put pieces of autoclave tape in each petri dishes and pots and I put all of them in the pressure cooker.
- I filled 1/3 of the pressure cooker with water
- I closed the autoclave (make sure it's perfectly closed), lock it and turn on the heat to the maximum
- It takes normally 10-20 min for the red pressure valve goes up (it's like a little button located on the lid of the autoclave)
- Then I turned the heat to the middle point and wait 15-20 min more
- I Released the pressure by keeping the valve up with a tweezers and then I opened the If there is black lines on the autoclave tape, the sterilization process is done !
Then, I let everything cool down to room temperature before the inoculation.
See below my inoculation set up:
To create a sterilised area, I turn on the gaz burner and spread some ethanol around it. Ethanol evaporates and this creates a sterile area between the table and the fire inside where you can work.
I filled all the petri dishes with the different kind of medium.
I first inoculated mycelium schizophilum in the 4 different kind of nutrients.
I then inoculated mycelium grey oyster in the 4 different kind of nutrients.
I finally inoculated tempeh starter in the 4 different kind of nutrients.
And I let them incubate at 26°C, and wait and see.
Evolution over the weeks ●
TextileLab contribution ●
Find below my contribution to the TextileLab biomaterial library of Waag:
→ get the .pdf file
For steps 1-2-3, read my post Grow Mycelium - making to know how to inoculate some mycelium into the liquid nutrient medium.
Mycelium and Straw
→ get the .pdf file
Read my project Mycelium, straw and weaving to see the process.
Body Incubator ●
Wear this necklace for 3 weeks and incubate edible mushrooms with the heat of your body. If you take well care of your micro pet, you may harvest some!!
- I bought small petri dishes in Pidiscat.cat
- I designed and 3D printed a petri dish holder
- I knotted a rope to go around the neck
I inoculated some grey oyster spawn in coffee ground. How to inoculate mycelium
I offered a few necklaces to my friends for the experience and the results were fruitful.